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Perform midline incision through skin from tip of xiphoid cartilage to groin, and then dissect percutaneous tissue free of underlying musculature laterally near incision Cut through peritoneum from the groin to xiphoid, and perform lateral cuts perpendicular to the midline through skin and abdominal fascia in order to further open the abdominal cavity (f) Use cotton-tipped applicators moistened with KRB-EDTA to push aside the intestines and stomach Move the liver gently, using a rolling motion with the applicators, until the portal vein is exposed (g) Loosely loop a suture around the portal vein near the hilus proximal to vessel branching and another one distally between the mesenteric veins (h) Insert the catheter into the portal vein, and position just beyond the proximal suture Attach the perfusate line to the catheter, and immediately cut the inferior vena cava with a scalpel.

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It is imperative to establish ow quickly, to prevent clotting within the liver Tie the distal suture, and then tie the proximal suture (i) Cut through the left-side rib cage to access the heart, and sever the left ventricle so that the heart stops Cut through the sternum along the midline to the clavicles, and cut along the diaphragm and ribs on both sides to create room for the liver to expand Turn off iso urane (j) Trim connective tissue between liver and surrounding organs Prop up liver with cotton-tipped applicators so that the liver is not stretched, to permit profuse ow of collagenase When the reservoir is almost emptied, add in the collagenase Allow the collagenase to perfuse the liver at 18 ml/min.

(k) Turn off the pump after deep ssures appear in the liver lobes, but before disintegration of the liver capsule and before the collagenase empties (approximately 11 min) Cut the catheter Pick up the liver with forceps and sever connective tissue to completely free liver from the abdominal cavity Place liver in Petri dish with KRB and take to laminar ow hood..

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This term is compatible with our de nition of a component or subsystem (COS) from 1, but it also contains some platform-speci c details In particular, the Microsoft NET Framework considers all components to be managed components or unmanaged components Managed components are compiled into the Intermediate Language (IL) format and execute within the Common Language Runtime (CLR) using the Common Language Speci cation (CLS), Common Type System (CTS), and Base Class Library (BCL) as we discussed in the previous sections Unmanaged components are not compiled into the IL format Each of the traditional programs we alluded to in Section 18115 is considered as an unmanaged component All code that is not based on the Microsoft NET Framework is considered traditional in this respect It is also possible to create unmanaged components that are based on the Microsoft .

should be remarked that we should not expect real-world data to behave this nicely. The presence of sampling error and other sources of noise will usually render our decisions about normality less clear-cut than this. Note the AD statistic and p-value reported by Minitab in each of Figures 2.9 to 2.11. This refers to the Anderson Darling test for normality. Smaller values of the AD statistic indicate that the normal distribution is a better t for the data. The null hypothesis is that the normal distribution ts, so that small p-values will indicate lack of t. Note that for the uniform and chi-square examples, the p-value for the AD test is

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Private Sub Workbook_BeforeClose(Cancel As Boolean) Dim Msg As String If Me.Saved Then Call DeleteMenu Exit Sub Else Msg = Do you want to save the changes you made to Msg = Msg & Me.Name & Ans = MsgBox(Msg, vbQuestion + vbYesNoCancel) Select Case Ans Case vbYes Me.Save Call DeleteMenu Case vbNo Me.Saved = True Call DeleteMenu Case vbCancel Cancel = True End Select End If End Sub

B12 (Ch3)

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