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22,23 The puri cation of pneumocandin analogues, a class of natural lipopeptides with antifungal activity, has been carried out using normal phase preparative HPLC24 The silica column with a ternary solvent system of EtOAc MeOH H2O gave a better resolution between the desire product B and the impurities A and C than reverse-phase HPLC The product solubility and the resolution are affected by the percentage of MeOH and H2O in the mobile phase The optimum solvent composition is 84 : 9 : 7 of EtOAc : MeOH : H2O The recovery yield of B is a function of the ow rate and the sample loading There is a trade-off between the recovery and the puri cation speed The puri cation speed is increased fourfold when achieving an 82% recovery compared with achieving a 100% recovery.

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